1NXX
MicArec pH 5.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.756, 92.416, 36.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.1991 |
| Rwork | 0.195 |
| R-free | 0.23291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxt |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.737 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 10590 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 95.0 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 40%v/v PEG 400, 200mM Mgcl2, 100mM citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
