1NXV
MicArec pH 4.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.448 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.689, 92.571, 37.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.220 - 2.000 |
| R-factor | 0.20791 |
| Rwork | 0.202 |
| R-free | 0.26857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.861 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 9309 | |
| Completeness [%] | 98.7 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | Phophate/citrate, PEG 300, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
