1NXT
MicArec pH 4.0
Experimental procedure
| Experimental method | SAD using Sulphur and Xenon |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.8 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.673, 92.829, 36.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.860 - 2.340 |
| R-factor | 0.21614 |
| Rwork | 0.214 |
| R-free | 0.23760 * |
| Structure solution method | SAD Xenon/Sulphur |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.838 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.860 | 2.402 |
| High resolution limit [Å] | 2.340 | 2.342 |
| Rmerge | 0.052 * | 0.116 * |
| Total number of observations | 110950 * | |
| Number of reflections | 5398 | |
| <I/σ(I)> | 11.6 | 5.1 |
| Completeness [%] | 99.0 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | 40%v/v PEG 300, 100mM Phosphate Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 25 (mM) | |
| 2 | 1 | drop | 300 (mM) | pH7.5 | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | PEG300 | 30 (%(v/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
