1NXS
MicArec pH4.9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-05 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.024 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.042, 92.581, 36.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.470 - 1.920 |
| R-factor | 0.19484 |
| Rwork | 0.191 |
| R-free | 0.23383 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxt |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.237 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.470 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 10912 | |
| Completeness [%] | 99.9 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG 300, Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
