1NXJ
Structure of Rv3853 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84520 |
| Spacegroup name | P 63 |
| Unit cell lengths | 102.462, 102.462, 117.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 * - 1.900 |
| Rwork | 0.190 |
| R-free | 0.22000 * |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.312 |
| Number of reflections | 54630 * | |
| <I/σ(I)> | 14.4 | 3.1 |
| Completeness [%] | 99.3 | 96.1 |
| Redundancy | 3.35 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 18 * | 0.45M sodium potassium tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | 140 (mM) | pH8.0 | |
| 3 | 1 | drop | protein | 2 (mg/ml) | |
| 4 | 1 | reservoir | potassium/sodium tartrate tetrahydrate | 0.45 (M) |
