1NWS
Crystal structure of human cartilage gp39 (HC-gp39) in complex with chitobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 43 |
Unit cell lengths | 128.220, 128.220, 109.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.700 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.087 * | 0.480 * |
Total number of observations | 148105 * | |
Number of reflections | 48088 | |
<I/σ(I)> | 11.4 | 2.5 |
Completeness [%] | 96.0 | 92.8 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 * | 277 | PEG8000, NaCl, Na-citrate buffer, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.10 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | 1 (M) | ||
3 | 1 | drop | BES | 10 (mM) | pH7.2 |
4 | 1 | reservoir | PEG8000 | 10 (%) | |
5 | 1 | reservoir | 0.5 (M) | ||
6 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.1 |