1NWR

Crystal structure of human cartilage gp39 (HC-gp39)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	120
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43
Unit cell lengths	127.300, 127.300, 107.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.600 *
R-factor	0.212
Rwork	0.212
R-free	0.24000 *
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	23.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600	2.600
Rmerge	0.060 *	0.259
Total number of observations	205355 *
Number of reflections	52490
<I/σ(I)>	18.2	4.1
Completeness [%]	99.6 *	96.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *	277	PEG8000, NaCl, Na-citrate buffer, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.10

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop		1 (M)
3	1	drop	BES	10 (mM)	pH7.2
4	1	reservoir	PEG8000	10 (%)
5	1	reservoir		0.5 (M)
6	1	reservoir	sodium citrate	0.1 (M)	pH5.1