1NWM

GAT domain of human GGA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	95
Detector technology	CCD
Collection date	2002-12-15
Detector	MARRESEARCH
Wavelength(s)	0.9199
Spacegroup name	H 3
Unit cell lengths	76.660, 76.660, 60.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.100 * - 2.400
R-factor	0.25369
Rwork	0.252
R-free	0.28600 *
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.330 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.055	0.296
Number of reflections	5195	474 *
<I/σ(I)>	22.6
Completeness [%]	98.7 *	90.1
Redundancy	3.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6 *	25 *	polyethylene glycol 4000, iso-propanol, sodium citrate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium citrate	0.1 (M)	pH5.6
3	1	reservoir	PEG4000	20 (%)
4	1	reservoir	isopropyl alcohol	20 (%)