1NWK
CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9799 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.216, 37.368, 85.027 |
| Unit cell angles | 90.00, 108.13, 90.00 |
Refinement procedure
| Resolution | 24.400 * - 1.850 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IJ6Z |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO (1.97.2) |
| Data scaling software | SCALEPACK (1.97.2) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.400 | 1.970 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.091 | 0.290 |
| Number of reflections | 28512 | |
| <I/σ(I)> | 12.1312 | 7.2 |
| Completeness [%] | 98.2 | 90.2 |
| Redundancy | 7.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 3350, CaCl2, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 393K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9-12 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 18 (%) | |
| 3 | 1 | reservoir | ACES | 9 (mM) | pH7.0 |
| 4 | 1 | reservoir | 365 (mM) | ||
| 5 | 1 | reservoir | dimethyl sulfoxide | 9 (%) |
