1NV9
HemK, apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.897, 59.013, 63.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.350* |
| R-factor | 0.24166 |
| Rwork | 0.239 |
| R-free | 0.30000 * |
| Structure solution method | MAD |
| Starting model (for MR) | 1nv8 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.039 | 0.404 |
| Total number of observations | 100547 * | |
| Number of reflections | 24661 * | |
| <I/σ(I)> | 20 | 2.3 |
| Completeness [%] | 97.7 | 79.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | PEG3000, Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 19K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | PEG3000 | 16-20 (%) | |
| 3 | 1 | reservoir | sodium citrate trihydrate | 100 (mM) | pH5.5 |
