1NV7
Fructose-1,6-Bisphosphatase Complex With AMP, Magnesium, Fructose-6-Phosphate, Phosphate and Thallium (20 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-21 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.950, 166.150, 79.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.150 |
| R-factor | 0.189 |
| Rwork | 0.182 |
| R-free | 0.25110 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.104 | |
| Total number of observations | 293146 * | |
| Number of reflections | 40229 | |
| Completeness [%] | 99.9 | 99.9 * |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG 3350, HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | PEG3350 | 8 (%(w/v)) | |
| 2 | 1 | drop | KPi | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | F6P | 5 (mM) | |
| 5 | 1 | drop | AMP | 5 (mM) | |
| 6 | 1 | drop | thalium acetate | 20 (mM) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 8 | 1 | reservoir | t-butyl alcohol | 5 (%) | |
| 9 | 1 | reservoir | glycerol | 27 (%(v/v)) |
