1NV4
Fructose-1,6-Bisphosphatase Complex with Magnesium, Fructose-6-Phosphate, Phosphate, EDTA and Thallium (1 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5415 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.074, 82.829, 165.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.184 |
| R-free | 0.23660 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.800 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.024 | |
| Total number of observations | 182349 * | |
| Number of reflections | 26022 | |
| Completeness [%] | 89.0 | 48.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG 3350, HEPES , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | PEG3350 | 8 (%(v/v)) | |
| 2 | 1 | drop | KPi | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | F6P | 5 (mM) | |
| 5 | 1 | drop | thalium acetate | 1 (mM) | |
| 6 | 1 | drop | EDTA | 0.2 (mM) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | t-butyl alcohol | 5 (%) | pH7.0 |
| 9 | 1 | reservoir | glycerol | 27 (%(v/v)) |
