1NV3
Fructose-1,6-Bisphosphatase Complex with Magnesium, Fructose-6-Phosphate, Phosphate and Thallium (100 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.361, 82.765, 165.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.21 |
| Rwork | 0.204 |
| R-free | 0.26610 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyi |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | |
| Total number of observations | 97976 * | |
| Number of reflections | 20719 | |
| Completeness [%] | 82.8 | 49.2 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG 3350, HEPES , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | KPi | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | F6P | 5 (mM) | |
| 5 | 1 | drop | thalium acetate | 100 (mM) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 7 | 1 | reservoir | t-butyl alcohol | 5 (%) | |
| 8 | 1 | reservoir | glycerol | 27 (%(v/v)) | |
| 9 | 1 | reservoir | PEG3350 | 8 (%(w/v)) |
