1NUW
Fructose-1,6-Bisphosphatase Complex with Magnesium, Fructose-6-Phosphate and Phosphate at pH 9.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Detector technology | CCD |
| Collection date | 2001-06-24 |
| Detector | SBC |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.600, 83.017, 165.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.168 |
| Rwork | 0.164 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyi |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 0.031 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.024 | |
| Total number of observations | 347535 * | |
| Number of reflections | 83972 * | |
| Completeness [%] | 92.6 | 65.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 295 | PEG 3350, Glycine, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | KPi | 10 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | F16P2 | 5 (mM) | |
| 5 | 1 | reservoir | glycine | 100 (mM) | pH9.6 |
| 6 | 1 | reservoir | t-butyl alcohol | 5 (%) | |
| 7 | 1 | reservoir | glycerol | 25 (%(v/v)) | |
| 8 | 1 | reservoir | PEG3350 | 10 (%(w/v)) |
