1NUB
HELIX C DELETION MUTANT OF BM-40 FS-EC DOMAIN PAIR
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.860, 88.140, 153.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.800 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BM-40 FS/EC DOMAIN PAIR WITH RESIDUES 168-208 REMOVED |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.920 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.106 | 0.445 |
Total number of observations | 98015 * | |
Number of reflections | 17878 | |
<I/σ(I)> | 6.6 | 1.8 |
Completeness [%] | 99.2 | 99.5 * |
Redundancy | 5.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | protein solution was mixed with an equal volume of reservoir solution in the drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | reservoir | PEG4000 | 12-14 (%(w/v)) | |
5 | 1 | reservoir | 2-propanol | 10 (%(v/v)) | |
6 | 1 | reservoir | Na-HEPES | 100 (mM) |