1NU0

Structure of the double mutant (L6M; F134M, SeMet form) of yqgF from Escherichia coli, a hypothetical protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97240
Spacegroup name	P 21 21 21
Unit cell lengths	58.500, 59.000, 67.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.600
Rwork	0.189
R-free	0.22800
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.019
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.046	0.161
Number of reflections	31128
<I/σ(I)>	28.1
Completeness [%]	99.6	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.25	298	24% polyethylene glycol monomethyl ether 2000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K