1NU0
Structure of the double mutant (L6M; F134M, SeMet form) of yqgF from Escherichia coli, a hypothetical protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97240 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.500, 59.000, 67.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.189 |
| R-free | 0.22800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.161 |
| Number of reflections | 31128 | |
| <I/σ(I)> | 28.1 | |
| Completeness [%] | 99.6 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 298 | 24% polyethylene glycol monomethyl ether 2000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
