1NT4
Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2146, 1.2149, 1.3190 |
| Spacegroup name | H 3 |
| Unit cell lengths | 156.263, 156.263, 84.575 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.420 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 * | 0.271 * |
| Total number of observations | 95761 * | |
| Number of reflections | 27760 * | |
| <I/σ(I)> | 17.4 | 3.5 |
| Completeness [%] | 91.9 | 91.1 |
| Redundancy | 3.45 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 * | 293 | Ammonium sulfate, PEG 500 mme, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.75-1.2 (M) | |
| 3 | 1 | reservoir | PEG5000 MME | 25 (%(w/v)) | |
| 4 | 1 | reservoir | MES | 0.05 (M) | pH6.25 |
