1NSY

CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-04-05
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	53.300, 87.800, 61.400
Unit cell angles	90.00, 110.60, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.174 *
Rwork	0.174
R-free	0.23000
RMSD bond length	0.015
RMSD bond angle	1.150
Data reduction software	MOSFLM
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.064
Total number of observations	177041 *
Number of reflections	33464
Completeness [%]	94.0
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.2 *	28 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	PEG400	22 (%(v/v))
3	1	2		0.05 (M)
4	1	2	sodium acetate	0.1 (M)