1NSR

Crystal structure of galactose mutarotase from Lactococcus lactis mutant D243N complexed with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	2002-09-26
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	44.900, 76.500, 211.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.181
Rwork	0.177
R-free	0.23300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1l7j
RMSD bond length	0.012
RMSD bond angle	2.220 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.049 *
Number of reflections	64992
<I/σ(I)>	16.7	2.6
Completeness [%]	94.8	82.5
Redundancy	2.5	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	4 *	MES, methylether PEG-5000, NaCl, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	15-19 (%)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	drop	enzyme	17.5 (mg/ml)