1NSP

MECHANISM OF PHOSPHATE TRANSFER BY NUCLEOSIDE DIPHOSPHATE KINASE: X-RAY STRUCTURES OF A PHOSPHO-HISTIDINE INTERMEDIATE OF THE ENZYMES FROM DROSOPHILA AND DICTYOSTELIUM

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	75.160, 75.160, 105.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.188
Rwork	0.188
RMSD bond length	0.014
RMSD bond angle	1.670
Phasing software	X-PLOR
Refinement software	X-PLOR (0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Rmerge	0.045 *
Total number of observations	74522 *
Number of reflections	10810
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	5 (%)
2	1	drop		20 (mM)
3	1	drop	Tris-HCl	50 (mM)
4	1	reservoir	PEG6000	10 (%)
5	1	reservoir	Tris-HCl	50 (mM)