1NSN

THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992-11
Detector	SIEMENS
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	234.700, 43.500, 74.400
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	5.000 - 2.800
R-factor	0.195
Rwork	0.195
R-free	0.28400 *
RMSD bond length	0.013
RMSD bond angle	27.000 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000
High resolution limit [Å]	2.700	2.770 *
Rmerge	0.048	0.103 *
Total number of observations	32555 *
Number of reflections	14626
Completeness [%]	81.0 *	25 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Chang, C.Y., (1994) J.Mol.Biol., 239, 154. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-maleic acid	100 (mM)
2	1	reservoir	sodium citrate	1.2 (M)
3	1	drop	protein	9 (mg/ml)