1NSM
Crystal structure of galactose mutarotase from Lactococcus lactis mutant D243A complexed with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 2002-05-01 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.800, 76.300, 211.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.23600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1l7j |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.560 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.043 * | |
| Number of reflections | 56391 | |
| <I/σ(I)> | 16.4 | 3.6 |
| Completeness [%] | 89.7 | 78.5 |
| Redundancy | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 4 * | MES, methylether PEG-5000, NaCl, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 15-19 (%) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 3 | 1 | drop | enzyme | 17.5 (mg/ml) |
