1NSL

Crystal structure of Probable acetyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Detector	SBC-2
Wavelength(s)	0.984
Spacegroup name	P 1 21 1
Unit cell lengths	59.339, 134.185, 91.062
Unit cell angles	90.00, 104.08, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.25808
Rwork	0.256
R-free	0.28550 *
Structure solution method	MAD
RMSD bond length	0.021 *
RMSD bond angle	2.337 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.450
Rmerge	0.102 *
Number of reflections	48725 *
Completeness [%]	100.0 *
Redundancy	2.40 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG6000	10 (%)
3	1	reservoir	sodium potassium phosphate		pH6.0
4	1	reservoir		0.1 (M)