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1NSJ

CRYSTAL STRUCTURE OF PHOSPHORIBOSYL ANTHRANILATE ISOMERASE FROM THERMOTOGA MARITIMA

Experimental procedure
Source typeROTATING ANODE
Source detailsELLIOTT GX-18
Temperature [K]277
Detector technologyIMAGE PLATE
Collection date1995-01-09
DetectorMARRESEARCH
Spacegroup nameP 61 2 2
Unit cell lengths68.400, 68.400, 185.400
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution10.000

*

- 2.000
R-factor0.192
Rwork0.192
R-free0.24300
Structure solution methodMULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length0.011
RMSD bond angle2.400
Data reduction softwareMOSFLM
Data scaling softwareCCP4 ((SCALA))
Phasing softwareX-PLOR (3.1)
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.110
High resolution limit [Å]2.0002.000
Rmerge0.0640.311
Total number of observations118018

*

Number of reflections17903
<I/σ(I)>8.62.4
Completeness [%]98.896.2
Redundancy6.64.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.54

*

drop solution was mixed with an equal volume of reservoir solution

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein19.4 (mg/ml)
21droppotassium phosphate50 (mM)
31dropEDTA1 (mM)
41dropdithiothreitol0.4 (mM)
51reservoirammonium sulfate1.5-2.1 (M)
61reservoir10 (mM)
71reservoirMES0.1 (M)

238268

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