1NSE
BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.000, 106.550, 156.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.206 |
| R-free | 0.27800 |
| Structure solution method | MAD+MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.021 |
| Data reduction software | DPS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 * | 0.175 * |
| Total number of observations | 217080 * | |
| Number of reflections | 74337 | |
| <I/σ(I)> | 19.3 | 3.5 |
| Completeness [%] | 96.5 | 80 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 15 (%) | |
| 2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 4 | 1 | drop | SEITU | 1 (mM) | can be replaced by 5mM L-Arg |
| 5 | 1 | drop | SDS | 0.035 (mM) | |
| 6 | 1 | drop | TCEP | 5 (mM) | can be replaced by 5mM glutathione sulfonate |
