1NSA

THREE-DIMENSIONAL STRUCTURE OF PORCINE PROCARBOXYPEPTIDASE B: A STRUCTURAL BASIS OF ITS INACTIVITY

Experimental procedure

Temperature [K]	277
Detector technology	TV AREA DETECTOR
Detector	BRUKER NONIUS FAST
Spacegroup name	P 43
Unit cell lengths	103.100, 103.100, 46.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.169 *
Rwork	0.169
RMSD bond length	0.015 *
RMSD bond angle	2.400 *
Data reduction software	ABSCOR
Data scaling software	ABSCOR
Refinement software	EREF

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300	2.300 *
Rmerge	0.090
Total number of observations	115799 *
Number of reflections	23217
Completeness [%]	93.7	72.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5	4 *	1.5 M AMMONIUM SULFATE AT PH5, pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)