1NR9
Crystal Structure of Escherichia coli 1262 (APC5008), Putative Isomerase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-06 |
Detector | SBC-2 |
Wavelength(s) | 0.97922, 0.97936,0.94644 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.373, 83.546, 94.896 |
Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
Resolution | 47.380 - 2.700 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.27500 |
Structure solution method | autoSHARP |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | autoSHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.380 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 0.446 |
Number of reflections | 26031 | |
<I/σ(I)> | 6.2 | |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | Potassium/Sodium Tartrate, Sodium Citrate, Ammonium Sulfate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |