1NR1

Crystal structure of the R463A mutant of human Glutamate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-06-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	96.920, 98.640, 124.260
Unit cell angles	86.48, 69.69, 60.87

Refinement procedure

Resolution	20.000 * - 3.300
R-factor	0.222
Rwork	0.222
R-free	0.27600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017 *
RMSD bond angle	1.980 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	3.450
High resolution limit [Å]	3.300	3.300
Rmerge	0.032 *	0.137 *
Number of reflections	50903
Completeness [%]	64.6 *	62.9 *
Redundancy	1.4 *	1.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	sodium phosphate, PEG 8000, BOG, sodium chloride, MPD, sodium azide, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	0.1 (M)	pH6.8
2	1	drop	protein	26 (mg/ml)
3	1	reservoir	PEG8000	10 (%(w/v))
4	1	reservoir		0.1 (M)
5	1	reservoir	octyl-beta-glucopyranoside	1.3 (%(w/v))
6	1	reservoir	MPD	7.5 (%(v/v))
7	1	reservoir	sodium phosphate	0.1 (M)	pH6.8
8	1	reservoir	sodium azide	1 (mM)