1NR0
Two Seven-Bladed Beta-Propeller Domains Revealed By The Structure Of A C. elegans Homologue Of Yeast Actin Interacting Protein 1 (AIP1).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.845, 65.174, 69.240 |
| Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
| Resolution | 23.600 * - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.100 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 * | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.273 |
| Number of reflections | 61821 | |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 4.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 316 | PEG 8000, Mn chloride, cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 316K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-4 (mg/ml) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | |
| 3 | 1 | reservoir | PEG8000 | 20 (%) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | glycerol | 3 (%) | pH6.0 |
