1NQ3
Crystal structure of the mammalian tumor associated antigen UK114
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.035, 77.998, 192.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.188 |
| R-free | 0.22600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QD9 (serine model) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.270 |
| Number of reflections | 39547 | |
| <I/σ(I)> | 20.14 | 4.45 |
| Completeness [%] | 98.8 | 96 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 8000, potassium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 22 (%(w/v)) | |
| 3 | 1 | reservoir | potassium phosphate | 50 (mM) | pH5.6 |
