1NPF
MYOGLOBIN (HORSE HEART) WILD-TYPE COMPLEXED WITH NITRIC OXIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.437, 28.728, 62.976 |
| Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.25000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dwr |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.055 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.159 |
| Total number of observations | 34504 * | |
| Number of reflections | 17516 | 1687 * |
| <I/σ(I)> | 11.3 | 3.4 |
| Completeness [%] | 93.1 | 91.1 |
| Redundancy | 1.95 | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | Chu, K., (2000) Nature, 403, 921. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.7-1.8 (M) | |
| 3 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.4-3.6 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
