1NP8
18-k C-terminally trunucated small subunit of calpain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.040, 67.570, 75.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 20.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 * | 0.221 * |
| Total number of observations | 160962 * | |
| Number of reflections | 20619 | |
| <I/σ(I)> | 15.5 | 6.4 |
| Completeness [%] | 97.4 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 294 | PEG, cadmium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 30 (M) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | PIPES | 25 (mM) | pH7.0 |
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | drop | dithiothreitol | 10 (mM) |
