1NP7
Crystal Structure Analysis of Synechocystis sp. PCC6803 cryptochrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.284, 89.477, 121.687 |
| Unit cell angles | 90.00, 120.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dnp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | 0.311 * |
| Number of reflections | 88628 | |
| <I/σ(I)> | 16.5 | 2.9 |
| Completeness [%] | 96.5 | 95.8 |
| Redundancy | 2.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20-24 * | ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (M) | |
| 5 | 1 | drop | glycerol | 10 (%) | pH8.0 |
| 6 | 1 | reservoir | ammonium sulfate | 55 (%sat) | |
| 7 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
