1NP6
Crystal structure of Escherichia coli MobB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.400, 64.791, 54.241 |
| Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.21838 |
| Rwork | 0.215 |
| R-free | 0.28700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partial model of MobB previously solved by MAD |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.310 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.870 |
| Rmerge | 0.042 * | 0.273 |
| Total number of observations | 364026 * | |
| Number of reflections | 29445 | |
| <I/σ(I)> | 18.6 | 4.6 |
| Completeness [%] | 99.3 * | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 4000, ammonium sulphate, glycerol, magnesium chloride, GDP, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 5 (mM) | ||
| 2 | 1 | drop | GDP | 5 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 10.5 (%(w/v)) | |
| 6 | 1 | reservoir | glycerol | 15 (%(w/v)) | |
| 7 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
| 8 | 1 | drop | protein | 10 (mg/ml) |
