1NOX
NADH OXIDASE FROM THERMUS THERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.530, 60.600, 56.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.000 - 1.590 |
| R-factor | 0.1896 |
| Rwork | 0.182 |
| R-free | 0.20432 |
| Structure solution method | ISOMORPHOUS AND MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL FROM ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.031 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.680 | |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.071 | 0.071 |
| Number of reflections | 28064 | |
| <I/σ(I)> | 6.2 | 3.2 |
| Completeness [%] | 96.2 | 95.3 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.6 * | 12 * | 0.05 M MES-TRIS BUFFER PH 6.0, 26 % PEG 4000, 0.3 M NACL, 50 MM FMN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | NADH oxidase | 15 (mg/ml) | |
| 2 | 1 | reservoir | FAD | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.8 (M) | |
| 4 | 1 | reservoir | citrate-pjosphate | 0.1 (M) | |
| 5 | 1 | reservoir | 0.25 (M) |
