1NOV

NODAMURA VIRUS

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1992-09
Detector	FILM
Spacegroup name	P 1 21 1
Unit cell lengths	562.090, 354.130, 612.770
Unit cell angles	90.00, 110.89, 90.00

Refinement procedure

Resolution	8.000 - 3.500
R-factor	0.296
Rwork	0.296
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FLOCK HOUSE VIRUS
RMSD bond length	0.014
RMSD bond angle	26.800 *
Data reduction software	OSC (PROGRAM (PURDUE))
Data scaling software	OSC (PROGRAM (PURDUE))
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.700
High resolution limit [Å]	3.300	3.300
Rmerge	0.141
Number of reflections	1708846
<I/σ(I)>	11.7	8.5
Completeness [%]	49.9	39
Redundancy	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6		10 - 15 MICRO LITRES OF 7MG/ML OF VIRUS IN PHOSPHATE BUFFER MIXED WITH ONE VOLUME OF CITRATE BUFFER AND EQUILIBRATED VERSES 20 ML OF CITRATE BUFFER (0.24 - 0.28 M SODIUM CITRATE, PH ADJUSTED TO 6.0 WITH ACETIC ACID, OR 0.24 M POTASSIUM CITRATE PH 6.0, BOTH WITH 0.1% BETA-OCTYL GLUCOPYRANOSIDE. CRYSTALS GROWN FROM VAPOR DIFFUSION USING SITTING DROP METHOD., vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	phosphate
3	1	reservoir	sodium citrate	0.24-0.28 (M)