1NOF
THE FIRST CRYSTALLOGRAPHIC STRUCTURE OF A XYLANASE FROM GLYCOSYL HYDROLASE FAMILY 5: IMPLICATIONS FOR CATALYSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-03-01 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.368, 49.580, 91.009 |
| Unit cell angles | 90.00, 101.66, 90.00 |
Refinement procedure
| Resolution | 9999.000 * - 1.420 |
| R-factor | 0.108 |
| Rwork | 0.102 |
| R-free | 0.16100 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | SDMS (DETECTOR SYSTEM) |
| Data scaling software | SDMS |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.570 | 1.520 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.066 | 0.132 |
| Total number of observations | 206521 * | |
| Number of reflections | 58915 | |
| <I/σ(I)> | 10.17 | 4.02 |
| Completeness [%] | 90.6 | 61.9 * |
| Redundancy | 3.51 | 1.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | Keen, N.T., (1996) Mol. Plant-Microbe Interact., 9, 651. * |
