1NOC
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) COMPLEXED WITH TYPE I E. COLI CHLORAMPHENICOL ACETYL TRANSFERASE AND IMIDAZOLE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 147.500, 147.500, 147.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 2.700 - 2.600 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.059 * | 0.310 * |
| Total number of observations | 226448 * | |
| Number of reflections | 32117 | |
| <I/σ(I)> | 30.6 | 2.9 |
| Completeness [%] | 98.9 | 92 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 22 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | CAT | 5 (mg/ml) | |
| 3 | 1 | drop | HEPES | 20 (mM) | |
| 4 | 1 | drop | glycerol | 5 (%) | |
| 5 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 6 | 1 | drop | IM/M | 50 (mM) | |
| 7 | 1 | drop | 27.5 (%sat) | ||
| 8 | 1 | reservoir | IM/M | 100 (mM) | |
| 9 | 1 | reservoir | 55 (%sat) |
