1NOB

KNOB DOMAIN FROM ADENOVIRUS SEROTYPE 12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	99
Detector technology	CCD
Detector	BRANDEIS
Spacegroup name	P 1 21 1
Unit cell lengths	61.910, 104.820, 77.190
Unit cell angles	90.00, 96.90, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.241 *
Rwork	0.240
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.008
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.220
Number of reflections	27987
<I/σ(I)>	10
Completeness [%]	100.0	100
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7		26% PEG 3350, pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	26 (%)