1NO1

Structure of truncated variant of B.subtilis SPP1 phage G39P helicase loader/inhibitor protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-05
Detector	MARRESEARCH
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	88.900, 91.300, 48.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.20565
Rwork	0.204
R-free	0.23000 *
Structure solution method	MAD
RMSD bond length	0.016 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.054	0.262
Total number of observations	161039 *
Number of reflections	28352 *
<I/σ(I)>	13.3
Completeness [%]	93.0	81
Redundancy	5.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	290	Marians, K.J., (1992) Annu. Rev. Biochem., 61, 673. *