1NNX
Structure of the hypothetical protein ygiW from E. coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.802, 60.638, 54.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.940 - 1.450 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.048 | 0.334 |
| Number of reflections | 15321 | |
| <I/σ(I)> | 17.73 | 2.07 |
| Completeness [%] | 92.5 | 77.4 |
| Redundancy | 0.93 | 0.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4 | 294 | 1.25 M (NH4)2SO4, 0.1 M Na acetate, pH 2.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K, pH 2.40 |
