1NNW
hypothetical protein from Pyrococcus furiosus Pfu-1218608
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.06 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.140, 50.842, 64.403 |
| Unit cell angles | 83.70, 81.64, 81.77 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.21692 |
| Rwork | 0.216 |
| R-free | 0.23498 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.136 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 46373 | |
| <I/σ(I)> | 21 | 3.6 |
| Completeness [%] | 67.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 5.6 | 291 | PEG 4000, isopropanol, sodium citrate, pH 5.6, modified microbatch, temperature 291K |
