1NNO

CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	166.072, 88.110, 113.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.650
R-factor	0.209
Rwork	0.209
R-free	0.24380 *
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	25.640 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.710
High resolution limit [Å]	2.650	2.650
Rmerge	0.110 *	0.569 *
Total number of observations	361716 *
Number of reflections	44045
<I/σ(I)>	10.3	2.1
Completeness [%]	90.7	93.3
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.4 *		pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium potassium phosphate	2 (M)
2	1	1	Tris-HCl	50 (mM)