1NN7

Crystal Structure Of The Tetramerization Domain Of The Shal Voltage-Gated Potassium Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-19
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 4 21 2
Unit cell lengths	59.999, 59.999, 61.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.400 - 2.100
Rwork	0.230
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	poly-serine model of PDB entry 3KVT
RMSD bond length	0.006
RMSD bond angle	1.226
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.055 *
Total number of observations	163537 *
Number of reflections	7071 *
<I/σ(I)>	41.54	7.55
Completeness [%]	99.5	97
Redundancy	8.74	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	23 *	PEG 4000, Magnesium chloride, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	5 (mM)	pH7.5
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
4	1	reservoir		0.2 (M)
5	1	reservoir	PEG4000	5 (%)