1NMU

MBP-L30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	93
Detector technology	CCD
Collection date	2000-11-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	79.650, 118.390, 153.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 2.310
R-factor	0.223
Rwork	0.214
R-free	0.25440 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	22.018 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.380
High resolution limit [Å]	2.310	2.310
Rmerge	0.086 *	0.359 *
Total number of observations	708695 *
Number of reflections	65330
Completeness [%]	99.2	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2 *	22 *	Sodium Citrate, Tris, Sodium Chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	sodium citrate	10 (mM)	pH6.2
3	1	drop	maltose	1 (mM)
4	1	drop	sodium azide	0.02 (%(w/v))
5	1	reservoir	sodium citrate	50 (%sat)
6	1	reservoir	Tris	0.1 (M)	pH7.0