1NMU
MBP-L30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-11-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.650, 118.390, 153.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.310 |
| R-factor | 0.223 |
| Rwork | 0.214 |
| R-free | 0.25440 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.018 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.380 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.086 * | 0.359 * |
| Total number of observations | 708695 * | |
| Number of reflections | 65330 | |
| Completeness [%] | 99.2 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | 22 * | Sodium Citrate, Tris, Sodium Chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 10 (mM) | pH6.2 |
| 3 | 1 | drop | maltose | 1 (mM) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | reservoir | sodium citrate | 50 (%sat) | |
| 6 | 1 | reservoir | Tris | 0.1 (M) | pH7.0 |
