1NMT
N-MYRISTOYL TRANSFERASE FROM CANDIDA ALBICANS AT 2.45 A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 132.000, 166.500, 179.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.850 - 2.450 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25100 |
| Structure solution method | SIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 26.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.98) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.850 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.047 | 0.318 |
| Number of reflections | 72615 | |
| <I/σ(I)> | 13.9 | 2.4 |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 7 | 1 | reservoir | glycerol | 15 (%(v/v)) |
