1NMC

COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 15 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	287
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1995-07
Detector	PHOTON FACTORY
Spacegroup name	P 4 21 2
Unit cell lengths	144.400, 144.400, 227.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.500
R-factor	0.22
Rwork	0.220
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nca
RMSD bond length	0.011
RMSD bond angle	26.400 *
Data reduction software	PROTEIN
Data scaling software	PROTEIN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.104
Total number of observations	156708 *
Number of reflections	67425
Completeness [%]	80.0	70

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6		Malby, R.L., (1993) Proteins: Struct.,Funct., Genet., 16, 57. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	NC10scFv	5 (mg/ml)
2	1	drop	N9NA	10 (mg/ml)
3	1	drop	potassium phosphate	1.7 (M)
4	1	reservoir	phosphate	1.3 (M)