1NLV

Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	95
Detector technology	CCD
Collection date	1999-04-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.07018
Spacegroup name	C 1 2 1
Unit cell lengths	178.446, 69.089, 56.570
Unit cell angles	90.00, 104.50, 90.00

Refinement procedure

Resolution	23.000 - 1.800
Rwork	0.210
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dag
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.338
Number of reflections	61207
Completeness [%]	99.9	99.4
Redundancy	4.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	lithium chloride, magnesium chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K