1NLQ

The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	57.608, 60.089, 73.426
Unit cell angles	90.00, 90.79, 90.00

Refinement procedure

Resolution	100.000 * - 1.550 *
Rwork	0.234
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.030 *	0.265
Total number of observations	478081 *
Number of reflections	80828
<I/σ(I)>	39.5	6.7
Completeness [%]	97.3	94.5
Redundancy	5.9	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	PEG 400, sodium chloride, magnesium chloride, tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	PEG 400, sodium chloride, magnesium chloride, tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG400	30-33 (%)
3	1	reservoir		20 (mM)