1NLM
CRYSTAL STRUCTURE OF MURG:GLCNAC COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.703, 83.651, 146.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fok |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.330 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 * | 0.254 * |
| Total number of observations | 195355 * | |
| Number of reflections | 26835 | |
| Completeness [%] | 92.6 | 82.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 4000, NaMES, Triton X-100, DTT, UDP-GlcNAc, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | UDP-GlcNAc | 10 (mM) | |
| 3 | 1 | reservoir | sodium MES | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | PEG4000 | 10-14 (%) | |
| 5 | 1 | reservoir | TritonX-100 | 0.4 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) |
